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Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae

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Homologous Recombination

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2153))

Abstract

The ribosomal RNA (rDNA) sequence is the most abundant repetitive element in the budding yeast genome and forms a tandem cluster of ~100–200 copies. Cells frequently change their rDNA copy number, making rDNA the most unstable region in the budding yeast genome. The rDNA region experiences programmed replication fork arrest and subsequent formation of DNA double-strand breaks (DSBs), which are the main drivers of rDNA instability. The rDNA region offers a unique system to understand the mechanisms that respond to replication fork arrest as well as the mechanisms that regulate repeat instability. This chapter describes three methods to assess rDNA instability.

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Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan and from the Japan Society for the Promotion of Science (JSPS) (17H01443 to T.K. and 17K15160 and 18H04709 to M.S.), Uehara Memorial Foundation to M.S. and JST CREST Grant Number JPMJCR19S3, Japan to T.K.

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Authors and Affiliations

  1. Laboratory of Genome Regeneration, Institute for Quantitative Biosciences, University of Tokyo, Tokyo, Japan

    Mariko Sasaki & Takehiko Kobayashi

Authors
  1. Mariko Sasaki
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  2. Takehiko Kobayashi
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Corresponding author

Correspondence to Takehiko Kobayashi .

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Editors and Affiliations

  1. Centro Andaluz de Biologı´a Molecular, y Medicina Regenerativa, CABIMER, Universidad de Sevilla, Sevilla, Spain

    Andrés Aguilera

  2. Institut Curie, PSL Research University, CNRS, UMR3348, F-91405, Orsay, France

    Aura Carreira

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Sasaki, M., Kobayashi, T. (2021). Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae. In: Aguilera, A., Carreira, A. (eds) Homologous Recombination. Methods in Molecular Biology, vol 2153. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0644-5_28

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  • DOI: https://doi.org/10.1007/978-1-0716-0644-5_28

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0643-8

  • Online ISBN: 978-1-0716-0644-5

  • eBook Packages: Springer Protocols

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