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Detection of DSBs in C. elegans Meiosis

  • Tatiana García-MuseEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2153)

Abstract

Meiosis is a specialized reductional cell division responsible for the formation of gametes and the generation of genetic diversity. A fundamental feature of the meiotic process is the initiation of homologous recombination (HR) by the programmed induction of DNA double-strand breaks (DSBs). Caenorhabditis elegans is a powerful experimental organism, which is used to study meiotic processes mainly due to the germline that allows for visualization of sequential stages of meiosis. C. elegans meiosis-programed DSBs are resolved through HR; hence, the germline provides a suitable model to study DSB repair. Classically direct procedures to detect and study intermediate steps in DSB repair by HR in the nematode rely on germline immunofluorescence against the strand exchange protein RAD-51.

Key words

Double-strand breaks C. elegans RAD-51 Immunofluorescence Homologous recombination 

Notes

Acknowledgments

This work was supported by a Spanish Ministry of Science, Innovation and Universities grant (PGC2018-101099-B-I00). T.G-M. was the holder of postdoctoral grants from the CSIC JAE-Doc and the Junta de Andalucía Excellence Program (CVI-4567).

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© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMERUniversidad de Sevilla-CSIC-UPOSevilleSpain

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