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Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis

  • Miyuki Yamaguchi
  • James E. HaberEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2153)

Abstract

By using an inducible site-specific double-strand break (DSB) in budding yeast, it is possible to monitor—in real time—the repair of the break by homologous recombination. A method is described using an ectopic homologous donor sequence to repair an HO endonuclease-induced DSB. These gene conversion events can occur with or without crossing-over, the products of which are distinguished as different-sized restriction endonuclease fragments. The method of Southern blotting is described in detail.

Key words

Saccharomyces cerevisiae Budding yeast Double-strand break repair Gene conversion Crossing-over Southern blot 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Department of Biology, Rosenstiel Basic Medical Sciences Research CenterBrandeis UniversityWalthamUSA

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