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Automated Glycan Assembly of Plant Cell Wall Oligosaccharides

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The Plant Cell Wall

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2149))

Abstract

Synthetic cell wall oligosaccharides are promising molecular tools for investigating the structure and function of plant cell walls. Their well-defined structure and high purity prevents misinterpretations of experimental data, and the possibility to introduce chemical handles provides means for easier localization and detection. Automated glycan assembly as emerged has a powerful new method for the efficient preparation of oligosaccharide libraries. We recently made use of this technology to prepare a collection of plant cell wall glycans for cell wall research. In this chapter, detailed experimental procedures for the automated synthesis of oligosaccharides that are ready for use in biological assays are described.

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Author information

Author notes

  1. Fabian Pfrengle

    Present address: Department of Chemistry, University of Natural Resources and Life Sciences, Vienna, Austria

Authors and Affiliations

  1. Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany

    Fabian Pfrengle

Authors
  1. Fabian Pfrengle
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Corresponding author

Correspondence to Fabian Pfrengle .

Editor information

Editors and Affiliations

  1. Botany and Plant Science School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland

    Zoë A. Popper

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Pfrengle, F. (2020). Automated Glycan Assembly of Plant Cell Wall Oligosaccharides. In: Popper, Z. (eds) The Plant Cell Wall. Methods in Molecular Biology, vol 2149. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0621-6_28

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  • DOI: https://doi.org/10.1007/978-1-0716-0621-6_28

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0619-3

  • Online ISBN: 978-1-0716-0621-6

  • eBook Packages: Springer Protocols

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