Skip to main content
SpringerLink
Log in

A Novel Technique for the Detection of LncRNAs on Tissue Sections

  • Protocol
  • First Online:
Experimental Protocols in Biotechnology

Part of the book series: Springer Protocols Handbooks ((SPH))

  • 588 Accesses

Abstract

Long noncoding RNA (LncRNA) is a class of noncoding RNA that typically consists of over 200 nucleotides that do not encode proteins. It is thought that lncRNA accounts for most of the noncoding transcriptome in humans, as tens of thousands of lncRNA transcripts have been identified. However, only a few lncRNAs have been characterized in detail to date. It is known that they act as important regulators of gene expression and are thought to have multiple functions in cellular and developmental processes. In this chapter we will discuss about the technique for detecting lncRNA from tissue section.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Huarte M (2015) The emerging role of lncRNAs in cancer. Nat Med 21(11):1253–1261. https://doi.org/10.1038/nm.3981

    Article  CAS  PubMed  Google Scholar 

  2. Ferrè F, Colantoni A, Helmer-Citterich M (2016) Revealing protein-lncRNA interaction. Brief Bioinform 17(1):106–116. https://doi.org/10.1093/bib/bbv031

    Article  CAS  PubMed  Google Scholar 

  3. Schmitz SU, Grote P, Herrmann BG (2016) Mechanisms of long noncoding RNA function in development and disease. Cell Mol Life Sci 73(13):2491–2509. https://doi.org/10.1007/s00018-016-2174-5

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Poller W, Dimmeler S, Heymans S et al (2018) Non-coding RNAs in cardiovascular diseases: diagnostic and therapeutic perspectives. Eur Heart J 39(29):2704–2716. https://doi.org/10.1093/eurheartj/ehx165

    Article  CAS  PubMed  Google Scholar 

  5. Wang P (2018) The opening of Pandora’s box: an emerging role of long noncoding RNA in viral infections. Front Immunol 9:3138. https://doi.org/10.3389/fimmu.2018.03138

    Article  CAS  PubMed  Google Scholar 

  6. Sun M, Kraus WL (2015) From discovery to function: the expanding roles of long noncoding RNAs in physiology and disease. Endocr Rev 36(1):25–64. https://doi.org/10.1210/er.2014-1034

    Article  CAS  PubMed  Google Scholar 

  7. Lv Y, Huang S (2019) Role of non-coding RNA in pancreatic cancer. Oncol Lett 18(4):3963–3973. https://doi.org/10.3892/ol.2019.10758

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Fattahi S, Kosari-Monfared M, Golpour M et al (2019) LncRNAs as potential diagnostic and prognostic biomarkers in gastric cancer: a novel approach to personalized medicine. J Cell Physiol 235(4):3189–3206. https://doi.org/10.1002/jcp.29260

    Article  CAS  PubMed  Google Scholar 

  9. Youness RA, Gad MZ (2019) Long non-coding RNAs: functional regulatory players in breast cancer. Noncoding RNA Res 4(1):36–44. https://doi.org/10.1016/j.ncrna.2019.01.003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Tripathi MK, Doxtater K, Keramatnia F et al (2018) Role of lncRNAs in ovarian cancer: defining new biomarkers for therapeutic purposes. Drug Discov Today 23(9):1635–1643. https://doi.org/10.1016/j.drudis.2018.04.010

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Parsons C, Tayoun AM, Benado BD et al (2018) The role of long noncoding RNAs in cancer metastasis. J Cancer Metastasis Treat 4(4):19. https://doi.org/10.20517/2394-4722.2018.11

    Article  CAS  Google Scholar 

  12. Jiang M-C, Ni J-J, Cui W-Y, Wang B-Y, Zhuo W (2019) Emerging roles of lncRNA in cancer and therapeutic opportunities. Am J Cancer Res 9(7):1354–1366. http://www.ncbi.nlm.nih.gov/pubmed/31392074.

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Tripathi MK, Zacheaus C, Doxtater K et al (2018) Z probe, an efficient tool for characterizing long non-coding RNA in FFPE tissues. Noncoding RNA 4(3):1–10. https://doi.org/10.3390/ncrna4030020

    Article  CAS  Google Scholar 

  14. Amini Chermahini G, Rashnonejad A, Harper SQ (2019) RNAscope in situ hybridization-based method for detecting DUX4 RNA expression in vitro. RNA 25(9):1211–1217. https://doi.org/10.1261/rna.070177.118

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Xie F, Timme KA, Wood JR (2018) Using single molecule mRNA fluorescent in situ hybridization (RNA-FISH) to quantify mRNAs in individual murine oocytes and embryos. Sci Rep 8(1):1–11. https://doi.org/10.1038/s41598-018-26345-0

    Article  CAS  Google Scholar 

  16. Wang F, Flanagan J, Su N et al (2012) RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn 14(1):22–29. https://doi.org/10.1016/j.jmoldx.2011.08.002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Rühle F, Stoll M (2016) Long non-coding RNA databases in cardiovascular research. Genomics Proteomics Bioinformatics 14:191–199

    Article  Google Scholar 

  18. Freedman JE, Miano JM (2017) Challenges and opportunities in linking long noncoding RNAs to cardiovascular, lung, and blood diseases. Arterioscler Thromb Vasc Biol 37:21–25

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, USA

    Andrew E. Massey

  2. Department of Immunology and Microbiology, School of Medicine, University of Texas Rio, McAllen, TX, USA

    Andrew E. Massey, Manish K. Tripathi, Murali M. Yallapu, Meena Jaggi & Subhash C. Chauhan

Authors
  1. Andrew E. Massey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manish K. Tripathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Murali M. Yallapu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Meena Jaggi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Subhash C. Chauhan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Subhash C. Chauhan .

Editor information

Editors and Affiliations

  1. Chhatrapati Shahu Ji Maharaj University, Kanpur, Uttar Pradesh, India

    Neelima Gupta

  2. Department of Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, Uttar Pradesh, India

    Varsha Gupta

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Massey, A.E., Tripathi, M.K., Yallapu, M.M., Jaggi, M., Chauhan, S.C. (2020). A Novel Technique for the Detection of LncRNAs on Tissue Sections. In: Gupta, N., Gupta, V. (eds) Experimental Protocols in Biotechnology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0607-0_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0607-0_15

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0606-3

  • Online ISBN: 978-1-0716-0607-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation