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Enzyme-Linked Immunosorbent Assay Detection of Bacterial Wilt–Causing Ralstonia solanacearum

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Experimental Protocols in Biotechnology

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Abstract

Ralstonia solanacearum, a Gram-negative phytopathogen, is the causative agent of a devastating bacterial wilt disease in Solanaceae crops leading to severe agroeconomic losses. Detection of bacterial wilt causing Ralstonia solanacearum from infected plants and water or soil samples is critically required for the early diagnosis, prophylaxis, and treatment of the disease. Enzyme-linked immunosorbent assay (ELISA) is one of the most proficient and relevant methods to detect the phytopathogen because of the specific interaction of enzyme linked antibodies with pathobiogenic molecules. Immunosorbent assay is a microtiter plate–based protocol designed for detecting and quantifying infected plants/soil samples select pathogen or its pathogenicity factor. Serodiagnostic techniques are highly beneficial over traditional methods as they are fast, reliable, easy, and inexpensive. Methodologies to prepare sample culture, plant materials, and soil for the detection of bacterial wilt causing Ralstonia solanacearum; standardized ELISA protocols; and specific precautions to carry out the assay successfully are discussed in this chapter. The sensitivity of ELISA test for different samples is discussed in the validation and interpretation section.

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Authors and Affiliations

  1. Department of Microbiology, Institute of Biosciences and Biotechnology, CSJM University, Kanpur, India

    Pramila Devi Umrao, Vineet Kumar & Shilpa Deshpande Kaistha

Authors
  1. Pramila Devi Umrao
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  2. Vineet Kumar
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  3. Shilpa Deshpande Kaistha
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Editors and Affiliations

  1. Chhatrapati Shahu Ji Maharaj University, Kanpur, Uttar Pradesh, India

    Neelima Gupta

  2. Department of Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, Uttar Pradesh, India

    Varsha Gupta

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Umrao, P.D., Kumar, V., Kaistha, S.D. (2020). Enzyme-Linked Immunosorbent Assay Detection of Bacterial Wilt–Causing Ralstonia solanacearum. In: Gupta, N., Gupta, V. (eds) Experimental Protocols in Biotechnology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0607-0_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0607-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0606-3

  • Online ISBN: 978-1-0716-0607-0

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