Abstract
The cornea is the outermost transparent and refractive barrier surface of the eye necessary for vision. Development of the cornea involves the coordinated production of extracellular matrix, epithelial differentiation, and endothelial cell expansion to produce a highly transparent tissue. Here we describe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for multiple downstream applications which are currently unattainable using traditional in vitro techniques.
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Acknowledgements
This work was supported by the NIH EY026104 (SC) and R00EY024648 (KW).
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Foster, J.W., Wahlin, K.J., Chakravarti, S. (2020). A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture. In: Ahearne, M. (eds) Corneal Regeneration. Methods in Molecular Biology, vol 2145. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0599-8_5
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DOI: https://doi.org/10.1007/978-1-0716-0599-8_5
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