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Design and Analysis of Pharmacological Studies in Aging

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Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2144))

Abstract

Measuring lifespan of the model organism, Caenorhabditis elegans, in a 96-well format enables the screening of large chemical libraries to identify biologically active molecules. Furthermore, the wide availability of these animals with specific genetic mutations allows the identification of genes that influence lifespan, and by extension, age-related biological pathways. Here, we present a method for measuring the lifespan of C. elegans in 96-well microtiter plates to identify and study pharmacologically active molecules that extend lifespan. The format of this assay is readily adapted for automated liquid handling systems and imaging of phenotypes.

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Acknowledgments

This protocol was originally developed by Xiaolan Ye and Michael Petrascheck in the Laboratory of Linda Buck. It was then further optimized by Sunitha Rangaraju and Gregory M. Solis. The most current version presented here was prepared by K.J.C. and M.P. with the critical reading of Alan To and Anabel Perez. This work was supported by grant funding to M.P., provided by NINDS R21 NS107951.

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Authors and Affiliations

  1. Department of Molecular Medicine, Chemistry, and Neuroscience, The Scripps Research Institute, La Jolla, CA, USA

    Khalyd J. Clay & Michael Petrascheck

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  1. Khalyd J. Clay
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  2. Michael Petrascheck
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Corresponding author

Correspondence to Michael Petrascheck .

Editor information

Editors and Affiliations

  1. Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA

    Sean P. Curran

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Clay, K.J., Petrascheck, M. (2020). Design and Analysis of Pharmacological Studies in Aging. In: Curran, S. (eds) Aging. Methods in Molecular Biology, vol 2144. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0592-9_7

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  • DOI: https://doi.org/10.1007/978-1-0716-0592-9_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0591-2

  • Online ISBN: 978-1-0716-0592-9

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