Abstract
Studies of Alzheimer’s disease (AD) using experimental systems most often involve transgenic mouse models that are characterized by neural accumulation of β-amyloid protein (Aβ), which is widely hypothesized to have a key role in AD pathogenesis. Quantification of Aβ in transgenic mice typically is accomplished through both biochemical and histochemical approaches. In this chapter, we describe two techniques for the histological detection of Aβ, immunostaining with Aβ antibodies and staining with the amyloid dye thioflavin S, and its quantification using digital imaging.
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Acknowledgments
This work was supported by NIH grant AG058068 to C.J.P.
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Christensen, A., Pike, C.J. (2020). Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Disease. In: Curran, S. (eds) Aging. Methods in Molecular Biology, vol 2144. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0592-9_19
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DOI: https://doi.org/10.1007/978-1-0716-0592-9_19
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0592-9
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