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Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis

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Axon Degeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2143))

Abstract

Changes of energy metabolism in axons and their adjacent glia as well as alterations in metabolic axon–glia cross talk are emerging as central mechanistic components underlying axon degeneration. The analysis of extracellular flux with commercial metabolic analyzers greatly facilitates the measurement of key parameters of glycolytic and mitochondrial energy metabolism in cells and tissues. In this chapter, I describe a straightforward method to capture bioenergetic profiles of acutely isolated peripheral nerve segments using the Agilent Seahorse XFe24 platform.

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Acknowledgments

This work was supported by Empire State Development Corporation for Hunter James Kelly Research Institute Grants W753 and U446; Hunter’s Hope Foundation, and Muscular Dystrophy Association Grants 23648 and 292306. I am especially grateful to Kevin Bittman (Agilent Technologies) for instrument and supplies support.

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Authors and Affiliations

  1. Hunter James Kelly Research Institute, New York State Center of Excellence in Bioinformatics & Life Sciences (CBLS) RM B4-314, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA

    Bogdan Beirowski

  2. Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA

    Bogdan Beirowski

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  1. Bogdan Beirowski
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Correspondence to Bogdan Beirowski .

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Editors and Affiliations

  1. Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA

    Elisabetta Babetto

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Beirowski, B. (2020). Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis. In: Babetto, E. (eds) Axon Degeneration. Methods in Molecular Biology, vol 2143. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0585-1_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0585-1_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0584-4

  • Online ISBN: 978-1-0716-0585-1

  • eBook Packages: Springer Protocols

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