Abstract
Neutralizing antibodies against Zika virus (ZIKV) are a robust indicator of exposure to ZIKV. Here we describe the plaque reduction neutralization test (PRNT), which quantifies ZIKV-neutralizing antibodies (nAbs) by incubating infectious ZIKV with different dilutions of sera and applying the mixture to a susceptible Vero cell monolayer. After 4 days of incubation, neutral red dye is added to optimize the visualization of plaques caused by ZIKV cytopathic effect. Compared with controls, sera containing nAbs against ZIKV will demonstrate ≥90% reduction in the number of plaques observed. This protocol can be utilized for research applications and serological diagnostic testing with the use of additional controls.
Kristina Dimitrova and Emelissa J. Mendoza are co-first authors.
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Dimitrova, K., Mendoza, E.J., Mueller, N., Wood, H. (2020). A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies. In: Kobinger, G., Racine, T. (eds) Zika Virus. Methods in Molecular Biology, vol 2142. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0581-3_5
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DOI: https://doi.org/10.1007/978-1-0716-0581-3_5
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0581-3
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