Abstract
Fasciola hepatica has a heteroxenous complex life cycle that alternates between an invertebrate intermediate and a mammalian definitive host. The life cycle has five well-defined phases within their hosts and the environment: (1) eggs released from the vertebrate host to the environment and its subsequent development; (2) emergence of miracidia and their search and penetration into an intermediate snail host; (3) development and multiplication of larval stages within the snail; (4) emergence of cercariae and the encystment in metacercariae; and (5) ingestion of infective metacercariae by the definitive host and development to its adult form. Here we describe some protocols to obtain and maintain different developmental stages of F. hepatica in the laboratory for different applications (molecular/cellular biology studies, vaccination trials, etc.).
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References
Carmona C, Tort JF (2017) Fasciolosis in South America: epidemiology and control challenges. J Helminthol 91(2):99–109
Malatji MP, Pfukenyi DM, Mukaratirwa S (2019) Fasciola species and their vertebrate and snail intermediate hosts in east and southern Africa: a review. J Helminthol 94:1–11
Ashrafi K et al (2014) Fascioliasis: a worldwide parasitic disease of importance in travel medicine. Travel Med Infect Dis 12(6 Pt A):636–649
Jaja IF et al (2017) Financial loss estimation of bovine fasciolosis in slaughtered cattle in South Africa. Parasite Epidemiol Control 2(4):27–34
Jefferies HS, Dawes B (1960) Elucidation of the life-cycle of Fasciola hepatica. Nature 185:331–332
Kendall SB, Ollerenshaw CB (1963) The effect of nutrition on the growth of Fasciola hepatica in its snail host. Proc Nutr Soc 22:41–46
Nari A et al (1986) Estudio preliminar sobre el desarrollo de Limnaea viatrix D´ Orbigny (1835) en condiciones controladas de temperatura y humedad. Veterinaria 95:13–17
Kendall S (1953) The life history of Limnaea truncatula under laboratory conditions. J Helminthol 27:17–28
Sukhdeo M, Sukhdeo S (2004) Trematode behaviours and the perceptual worlds of parasites. Can J Zool 82:292–315
Nari A et al (1983) Efecto de la temperatura en el desarrollo de Fasciola hepática en su huésped intermediario Limnaea viatrix D´ Orbigny (1835). Veterinaria 19:36–39
Acknowledgments
We would like to thank Lic. Federico Fossa and Dr. Nicolas Dell’Oca for sharing images related to egg culture and snail infection. We also would like to thank Dr. Gabriel Rinaldi and Dr. Gabriela Maggioli for sharing images related to metacercarial excystment and adult parasite.
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Gayo, V., Cancela, M., Acosta, D. (2020). Maintenance of Life Cycle Stages of Fasciola hepatica in the Laboratory. In: Cancela, M., Maggioli, G. (eds) Fasciola hepatica. Methods in Molecular Biology, vol 2137. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0475-5_1
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DOI: https://doi.org/10.1007/978-1-0716-0475-5_1
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0475-5
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