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Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging

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Book cover Clinical and Preclinical Models for Maximizing Healthspan

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2138))

Abstract

This chapter describes a protocol for proteomic profiling of the rat hippocampal proteome using a combination of liquid chromatography tandem mass spectrometry (LC-MS/MS) and data analysis to determine the cellular location of the identified proteins. In the example given, many of these proteins were localized in the plasma membrane and nucleus. These could be of interest in further studies of neurological and neurodegenerative disorders linked with hippocampal dysfunction, such as aging, major depression, and Alzheimer’s disease.

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Author information

Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil

    Paul C. Guest & Daniel Martins-de-Souza

  2. Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK

    Hassan Rahmoune

  3. Instituto Nacional de Biomarcadores em Neuropsiquiatria (INBION), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), São Paulo, Brazil

    Daniel Martins-de-Souza

  4. UNICAMP Neurobiology Center, Campinas, Brazil

    Daniel Martins-de-Souza

Authors
  1. Paul C. Guest
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  2. Hassan Rahmoune
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  3. Daniel Martins-de-Souza
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Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Guest, P.C., Rahmoune, H., Martins-de-Souza, D. (2020). Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging. In: Guest, P. (eds) Clinical and Preclinical Models for Maximizing Healthspan. Methods in Molecular Biology, vol 2138. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0471-7_30

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  • DOI: https://doi.org/10.1007/978-1-0716-0471-7_30

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0470-0

  • Online ISBN: 978-1-0716-0471-7

  • eBook Packages: Springer Protocols

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