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Histomorphometric Analysis of Anti-Aging Properties on Rat Skin

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Clinical and Preclinical Models for Maximizing Healthspan

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2138))

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Abstract

We present a histomorphometric analysis to enable quantitative measurement of anti-aging activity of topical substances in rat skin. In this method, the measured parameters were closely related to changes that occur on the skin caused by exposure to UV light. We used this protocol to determine the anti-aging activities of an ethanolic extract of Curcuma heyneana rhizome and retinoic acid by a measurement of the changes in epidermal thickness, the number of sunburned cells, the number of fibroblasts, and the space between collagen fibers.

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Acknowledgments

This work was supported by an International Research Collaboration and Scientific Publication grant from the Ministry of Research, Technology, and Higher Education, Republic of Indonesia (Grant no. 597/UN3.14/LT/2017). Thanks to PT. Rembaka for the drug reference used in the in vivo assay. Also, thanks to all my student for the nice pictures.

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Authors and Affiliations

  1. Pharmacognosy and Phytochemistry Department, Faculty of Pharmacy, Airlangga University, Surabaya, East Java, Indonesia

    Idha Kusumawati, Kresma Oky Kurniawan & Subhan Rullyansyah

  2. Veterinary Anatomy Department, Faculty of Veterinary, Airlangga University, Surabaya, East Java, Indonesia

    Eka Pramyrtha Hestianah

Authors
  1. Idha Kusumawati
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  2. Kresma Oky Kurniawan
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  3. Subhan Rullyansyah
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  4. Eka Pramyrtha Hestianah
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Corresponding author

Correspondence to Idha Kusumawati .

Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Kusumawati, I., Kurniawan, K.O., Rullyansyah, S., Hestianah, E.P. (2020). Histomorphometric Analysis of Anti-Aging Properties on Rat Skin. In: Guest, P. (eds) Clinical and Preclinical Models for Maximizing Healthspan. Methods in Molecular Biology, vol 2138. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0471-7_22

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  • DOI: https://doi.org/10.1007/978-1-0716-0471-7_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0470-0

  • Online ISBN: 978-1-0716-0471-7

  • eBook Packages: Springer Protocols

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