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Coenzyme Q10 Assessment and the Establishment of a Neuronal Cell Model of CoQ10 Deficiency

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Part of the Methods in Molecular Biology book series (MIMB, volume 2138)

Abstract

Coenzyme Q10 (CoQ10) plays a key role as an electron carrier in the mitochondrial respiratory chain and as a cellular antioxidant molecule. A deficit in CoQ10 status may contribute to disease pathophysiology by causing a failure mitochondrial energy metabolism as well as compromising cellular antioxidant capacity. This chapter outlines the analytical methods used for determining cellular CoQ10 status using high-pressure liquid chromatography with ultraviolet (HPLC-UV) detection. In addition, we present a pharmacological procedure for establishing a human neuronal cell model of CoQ10 deficiency, for use in research studies.

Key words

Coenzyme Q10 CoQ10 Mitochondrial respiratory chain Antioxidant Disease Neuronal cell model 
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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.School of Pharmacy and Biomolecular SciencesLiverpool John Moores UniversityLiverpoolUK
  2. 2.Institute of NeurologyLondonUK

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