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Assays to Analyze Adhesion of Group A Streptococcus to Host Cells

Part of the Methods in Molecular Biology book series (MIMB,volume 2136)

Abstract

The critical first step of Group A Streptococcus (GAS) pathogenesis is adhesion to the host pharyngeal and skin epithelial cell surfaces (Brouwer et al., FEBS Lett 590:3739–3757, 2016). Host-cell adhesion assays provide a straightforward model to study these host–pathogen interactions. Here, we describe the culturing of immortalized cell lines into monolayers to mimic host epithelia. Various GAS strains can then be added to study their adhesion properties. In addition, we describe the use of antibodies raised against the cell-surface components of GAS to study if these are able to neutralize the binding of GAS to the cell lines. This provides an indication if these cell-surface components are involved in adhesion and if antibodies generated against them function through neutralization.

Key words

  • Host-cell adhesion
  • Immortalized cell lines
  • Antibodies

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  • DOI: 10.1007/978-1-0716-0467-0_20
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References

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Acknowledgments

This work was supported by the Health Research Council (HRC) New Zealand and the Maurice Wilkins Centre for Molecular Biodiscovery. J.M.S.L. is a Heart Foundation NZ research fellow.

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Correspondence to Thomas Proft or Jacelyn M. S. Loh .

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Khemlani, A.H.J., Proft, T., Loh, J.M.S. (2020). Assays to Analyze Adhesion of Group A Streptococcus to Host Cells. In: Proft, T., Loh, J. (eds) Group A Streptococcus. Methods in Molecular Biology, vol 2136. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0467-0_20

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  • DOI: https://doi.org/10.1007/978-1-0716-0467-0_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0466-3

  • Online ISBN: 978-1-0716-0467-0

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