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Quantum Dots pp 169–177Cite as

Labeling Neuronal Proteins with Quantum Dots for Single-Molecule Imaging

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2135))

Abstract

Single-molecule imaging has illuminated dynamics and kinetics of neuronal proteins in their native membranes helping us understand their effective roles in the brain. Here, we describe how nanometer-sized fluorescent semiconductors called quantum dots (QD) can be used to label neuronal proteins in a single QD imaging format. We detail two generalizable protocols accompanied by experimental considerations giving the user options in approach tailored to the materials and equipment available. These protocols can be modified for experiments to verify target specificity, as well as single molecule analysis such as single particle tracking and protein clustering.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Vanderbilt University, Nashville, TN, USA

    Lucas B. Thal, Oleg Kovtun & Sandra J. Rosenthal

  2. Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, TN, USA

    Lucas B. Thal & Sandra J. Rosenthal

  3. Department of Pharmacology, Vanderbilt University, Nashville, TN, USA

    Sandra J. Rosenthal

  4. Department of Physics and Astronomy, Vanderbilt University, Nashville, TN, USA

    Sandra J. Rosenthal

  5. Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA

    Sandra J. Rosenthal

Authors
  1. Lucas B. Thal
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  2. Oleg Kovtun
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  3. Sandra J. Rosenthal
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Corresponding author

Correspondence to Sandra J. Rosenthal .

Editor information

Editors and Affiliations

  1. Biomedical Nanotechnology Group, Federal University of Pernambuco, Recife, Pernambuco, Brazil

    Adriana Fontes

  2. Biomedical Nanotechnology Group, Federal University of Pernambuco, Recife, Pernambuco, Brazil

    Beate S. Santos

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Thal, L.B., Kovtun, O., Rosenthal, S.J. (2020). Labeling Neuronal Proteins with Quantum Dots for Single-Molecule Imaging. In: Fontes, A., Santos, B. (eds) Quantum Dots. Methods in Molecular Biology, vol 2135. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0463-2_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0463-2_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0462-5

  • Online ISBN: 978-1-0716-0463-2

  • eBook Packages: Springer Protocols

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