Plant Embryogenesis pp 269-282 | Cite as
Isolated Microspore Culture in Brassica napus
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Abstract
Isolated microspore culture is the most efficient technique among those used to induce microspore embryogenesis. In the particular case of Brassica napus, it is also the most widely used and optimized. In this chapter, we describe a protocol for microspore culture in B. napus which includes the steps necessary to isolate and culture microspores, to induce microspore-derived embryos, to produce doubled haploid plants from them, as well as to check for the developmental stage of the microspores isolated, their viability, and the ploidy level of regenerated plantlets.
Key words
Androgenesis Doubled haploid Embryogenesis Haploid Microspores Pollen Rapeseed Tissue cultureNotes
Acknowledgments
This work was supported by grant AGL2017-88135-R to J.M.S.S. from MINECO jointly funded by FEDER.
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