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Plant Embryogenesis pp 257–267Cite as

In Vitro Production of Zygotes by Electrofusion of Rice Gametes

Part of the Methods in Molecular Biology book series (MIMB,volume 2122)

Abstract

In angiosperms, fertilization and embryogenesis occur in the embryo sac, which is deeply embedded in ovular tissue. In vitro fertilization (IVF) systems using isolated gametes have been utilized to dissect postfertilization events in angiosperms, such as egg activation, zygotic development, and early embryogenesis. In addition, using IVF systems, interspecific zygotes and polyploid zygotes have been artificially produced, and their developmental profiles/mechanisms have been analyzed. Taken together, the IVF system can be considered a powerful technique for investigating the fertilization-induced developmental sequences in zygotes and generating new cultivars with desirable characteristics. Here, we describe the procedures for the isolation of rice gametes, electrofusion of gametes, and the culture of the produced zygotes and embryo.

Key words

  • Egg cell
  • Sperm cell
  • In vitro fertilization
  • Zygote
  • Embryo
  • Rice

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  • DOI: 10.1007/978-1-0716-0342-0_18
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Authors and Affiliations

  1. Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan

    Md Hassanur Rahman, Erika Toda & Takashi Okamoto

Authors
  1. Md Hassanur Rahman
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  2. Erika Toda
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  3. Takashi Okamoto
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Corresponding author

Correspondence to Takashi Okamoto .

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Editors and Affiliations

  1. Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany

    Dr. Martin Bayer

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Rahman, M.H., Toda, E., Okamoto, T. (2020). In Vitro Production of Zygotes by Electrofusion of Rice Gametes. In: Bayer, M. (eds) Plant Embryogenesis. Methods in Molecular Biology, vol 2122. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0342-0_18

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  • DOI: https://doi.org/10.1007/978-1-0716-0342-0_18

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