Leafhoppers are one of the most abundant groups of plant feeding insects in the world of family Cicadellidae of the order Homoptera. Nearly 25 genera with more than 50 species of different leafhoppers are reported to be vector of different plant viruses. All the leafhopper-borne viruses are transmitted in a semi-persistent or persistent circulative or persistent propagative manner. This chapter highlights materials and methods for raising leafhopper colonies and their utilization for virus transmission experiments. Specific methods for rearing of the Nephotettix virescens for the transmission of rice tungro spherical virus are discussed and explained in the chapter.
Key words
- Leafhopper
- Semi-persistent viruses
- Persistent viruses
- Persistent propagative viruses