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Introduction and Perspectives of DNA Electrophoresis

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DNA Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2119))

Abstract

Gel electrophoresis of DNA is one of the most frequently used techniques in molecular biology. Typically, it is used in the following: the analysis of in vitro reactions and purification of DNA fragments, analysis of PCR reactions, characterization of enzymes involved in DNA reactions, and sequencing. With some ingenuity gel electrophoresis of DNA is also used for the analysis of cellular biochemical reactions. For example, DNA breaks that accumulate in cells are analyzed by the comet assay and pulsed-field gel electrophoresis (PFGE). Furthermore, DNA replication intermediates are analyzed with two-dimensional (2D) gel electrophoresis. Moreover, several new methods for analyzing various chromosomal functions in cells have been developed. In this chapter, a brief introduction to these is given.

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Acknowledgments

I thank Dominic James, PhD, from Edanz Group (www.edanzediting.com/ac) for editing a draft of the manuscript.

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Authors and Affiliations

  1. Clinical Engineering Research Center, Faculty of Medicine, Oita University, Yufu, Oita, Japan

    Katsuhiro Hanada

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  1. Katsuhiro Hanada
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Correspondence to Katsuhiro Hanada .

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Editors and Affiliations

  1. Clinical Engineering Research Center, Faculty of Medicine, Oita University, Yufu, Oita, Japan

    Katsuhiro Hanada

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Hanada, K. (2020). Introduction and Perspectives of DNA Electrophoresis. In: Hanada, K. (eds) DNA Electrophoresis. Methods in Molecular Biology, vol 2119. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0323-9_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0323-9_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0322-2

  • Online ISBN: 978-1-0716-0323-9

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