Abstract
CD8+ T cells constitute an essential component of the adaptive immune system. They are activated through T cell receptor (TCR) recognizing antigenic peptides presented by MHC class I molecules expressed by antigen-presenting cells, such as dendritic cells (DCs). Harvesting a large number of activated, antigen-specific human CD8+ T cells for functional studies has been a laborious task for immunologists, largely because of the variables associated with DC preparations. Here we describe a robust, cost-effective DC-free antigen-presenting system capable of generating a large number of antigen-specific CD8+ T cells in vitro.
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Acknowledgments
This work was supported in part by the National Science Council grant (NSC 98-2320-B-007-004-MY3) and the Ministry of Science and Technology grants (MOST 106-2320-B-007-001; MOST 107-2320-B-007-002) to C.-C. C.
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Pang, YG., Chang, CC. (2020). Artificial Antigen Presentosomes for T Cell Activation. In: Liu, C. (eds) T-Cell Receptor Signaling. Methods in Molecular Biology, vol 2111. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0266-9_12
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DOI: https://doi.org/10.1007/978-1-0716-0266-9_12
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