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Immunity in Insects pp 79–95Cite as

Methods to Quantify In Vivo Phagocytic Uptake and Opsonization of Live or Killed Microbes in Drosophila melanogaster

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Abstract

Here we describe different phagocytosis assays in Drosophila, using various killed or live microbes (bacteria and fungi). Different ex vivo and in vivo approaches are shown, to quantify larval and adult phagocytosis of microorganisms by hemocytes. We also explain how to perform an in vivo opsonization assay. Altogether, these protocols represent a useful range of tools to the researcher interested in the detailed analysis of phagocytosis in the context of the study of host-pathogen relationships.

Key words

  • Opsonization
  • Phagocytosis
  • Drosophila
  • Larva
  • Hemocytes
  • Bacteria
  • Fungi
  • Yeast
  • Infection
  • pHrodo

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Acknowledgments

We would like to thank François Lapraz (Institute of Biology Valrose, Nice, France) and Gábor Csordás (Institute for Genetics, University of Cologne) for their advice for adult hemolymph collection and Marion Draheim for critical reading of the manuscript. DF’s teamwork at SFHI is partially funded through the 1,000 Talent Program of Global experts of China.

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Authors and Affiliations

  1. Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou, P. R. China

    Samuel Liégeois, Wenhui Wang & Dominique Ferrandon

  2. Université de Strasbourg CNRS, M3I UPR 9022, Strasbourg, France

    Samuel Liégeois, Wenhui Wang & Dominique Ferrandon

Authors
  1. Samuel Liégeois
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  2. Wenhui Wang
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  3. Dominique Ferrandon
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Corresponding author

Correspondence to Samuel Liégeois .

Editor information

Editors and Affiliations

  1. Department of Biology, University of Padova, Padova, Italy

    Federica Sandrelli

  2. Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy

    Gianluca Tettamanti

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Liégeois, S., Wang, W., Ferrandon, D. (2020). Methods to Quantify In Vivo Phagocytic Uptake and Opsonization of Live or Killed Microbes in Drosophila melanogaster. In: Sandrelli, F., Tettamanti, G. (eds) Immunity in Insects. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0259-1_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0259-1_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0258-4

  • Online ISBN: 978-1-0716-0259-1

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