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Analysis of IFNγ-Induced Migration of Ovarian Cancer Cells

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)

Abstract

IFNγ is a pleiotropic cytokine that has both antitumor functions and pro-tumorigenic effects. Recent studies have shown that IFNγ induces expression of the immune checkpoint PD-L1 in ovarian cancer (OC) cells, resulting in their increased proliferation and tumor growth. Here, we tested the hypothesis that IFNγ induces migration of OC cells. Using the scratch wound healing assay, our results demonstrate that IFNγ promotes OC cell migration, thus adding to the complexities of IFNγ pro-tumorigenic mechanisms. This chapter describes analysis of the IFNγ-induced migration of OC cells by the wound healing assay followed by quantification of the obtained images using ImageJ software.

Key words

Cell migration Cell proliferation Interferon-γ Ovarian cancer Scratch assay Wound healing assay 
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Notes

Acknowledgements

This work was supported by NIH grant CA202775 to I. Vancurova.

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Biological SciencesSt. John’s UniversityQueensUSA

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