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Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells

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Book cover Immune Mediators in Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2108))

Abstract

Interferon-γ (IFNγ) is a pleiotropic cytokine that signals to many different cell types. IFNγ has both antitumor functions and pro-tumorigenic effects and regulates different aspects of cell physiology, including metabolism. Cancer cells undergo a complex rearrangement of metabolic pathways that allows them to satisfy the needs of increased proliferation, and many cancer cells redirect glucose metabolism from oxidative phosphorylation to aerobic glycolysis. In this chapter, we describe a protocol that utilizes the Agilent Seahorse XFp Analyzer to assess mitochondrial respiration and glycolysis in ovarian cancer cells treated with IFNγ.

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Acknowledgment

This work was supported by NIH grant GM120710 to A. Vancura.

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Authors and Affiliations

  1. Department of Biological Sciences, St. John’s University, Queens, NY, USA

    Pritpal Kaur, Shreya Nagar, Madhura Bhagwat, Mohammad M. Uddin, Yan Zhu & Ales Vancura

Authors
  1. Pritpal Kaur
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  2. Shreya Nagar
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  3. Madhura Bhagwat
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  4. Mohammad M. Uddin
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  5. Yan Zhu
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  6. Ales Vancura
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Corresponding author

Correspondence to Ales Vancura .

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Editors and Affiliations

  1. Department of Biological Sciences, St. John’s University, Queens, NY, USA

    Ivana Vancurova

  2. Department of Biological Sciences, St. John’s University, Queens, NY, USA

    Yan Zhu

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Kaur, P., Nagar, S., Bhagwat, M., Uddin, M.M., Zhu, Y., Vancura, A. (2020). Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells. In: Vancurova, I., Zhu, Y. (eds) Immune Mediators in Cancer. Methods in Molecular Biology, vol 2108. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0247-8_17

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  • DOI: https://doi.org/10.1007/978-1-0716-0247-8_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0246-1

  • Online ISBN: 978-1-0716-0247-8

  • eBook Packages: Springer Protocols

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