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Transcription Inhibition by PNA-Induced R-Loops

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Peptide Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2105))

Abstract

R-loops are structures consisting of an RNA-DNA duplex and an unpaired DNA strand. They can form during transcription upon nascent RNA “threadback” invasion into the DNA duplex to displace the non-template DNA strand. R-loops occur naturally in all kingdoms of life, and they have multiple biological effects. Therefore, it is of interest to study the artificial induction of R-loops and to monitor their effects in model in vitro systems to learn mechanisms. Here we describe transcription blockage in vitro by R-loop formation induced by peptide nucleic acid (PNA) binding to the non-template DNA strand.

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Authors and Affiliations

  1. Department of Biology, Stanford University, Stanford, CA, USA

    Boris P. Belotserkovskii, Sum-yan Ng & Philip C. Hanawalt

Authors
  1. Boris P. Belotserkovskii
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  2. Sum-yan Ng
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  3. Philip C. Hanawalt
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Corresponding author

Correspondence to Philip C. Hanawalt .

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Editors and Affiliations

  1. Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Peter E. Nielsen

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Belotserkovskii, B.P., Ng, Sy., Hanawalt, P.C. (2020). Transcription Inhibition by PNA-Induced R-Loops. In: Nielsen, P. (eds) Peptide Nucleic Acids. Methods in Molecular Biology, vol 2105. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0243-0_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0243-0_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0242-3

  • Online ISBN: 978-1-0716-0243-0

  • eBook Packages: Springer Protocols

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