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Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding

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RNA Chaperones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2106))

Abstract

Cotranscriptional RNA folding plays important roles in gene regulation steps such as splicing, transcription termination, and translation initiation. Progression of our understanding of cotranscriptional RNA folding mechanisms is still retarded by the lacking of experimental tools to study the kinetics of cotranscriptional RNA folding properly. In this chapter, we describe fluorescence resonance energy transfer (FRET) assay that enables the study of RNA cotranscriptional folding at the single-molecule level.

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Acknowledgments

This work was supported by a Creative Research Initiative program (2009-0081562) to SH.

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Authors and Affiliations

  1. Department of Physics and Astronomy, Seoul National University, Seoul, Republic of Korea

    Heesoo Uhm & Sungchul Hohng

  2. Institute of Applied Physics, Seoul National University, Seoul, Republic of Korea

    Heesoo Uhm & Sungchul Hohng

Authors
  1. Heesoo Uhm
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  2. Sungchul Hohng
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Corresponding author

Correspondence to Sungchul Hohng .

Editor information

Editors and Affiliations

  1. Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, University Hospital of Regensburg, Regensburg, Germany

    Tilman Heise

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Uhm, H., Hohng, S. (2020). Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding. In: Heise, T. (eds) RNA Chaperones. Methods in Molecular Biology, vol 2106. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0231-7_17

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  • DOI: https://doi.org/10.1007/978-1-0716-0231-7_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0230-0

  • Online ISBN: 978-1-0716-0231-7

  • eBook Packages: Springer Protocols

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