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Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones

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RNA Chaperones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2106))

Abstract

The FinO family of proteins constitutes a group of RNA chaperones that interacts with small RNAs (sRNAs) to regulate gene expression in many bacterial species. Here we describe detailed protocols for the biochemical analysis of the RNA chaperone activity of these proteins. Methods are described for preparation of RNA, RNA 5′ end labeling with radioisotope and modified EMSA protocols to test the ability of these proteins to catalyze RNA strand exchange and RNA duplex formation.

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Acknowledgments

We would like to thank Dr. Alexandru Ghetu for early work establishing the strand exchange assay and duplexing assay in FinOP system. This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (NSERC; RGPIN-2016-05163).

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Authors and Affiliations

  1. Department of Biochemistry, University of Alberta, Edmonton, AB, Canada

    Hyeong Jin Kim, Steven Chaulk, David Arthur, Ross A. Edwards & J. N. Mark Glover

Authors
  1. Hyeong Jin Kim
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  2. Steven Chaulk
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  3. David Arthur
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  4. Ross A. Edwards
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  5. J. N. Mark Glover
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Corresponding author

Correspondence to J. N. Mark Glover .

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Editors and Affiliations

  1. Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, University Hospital of Regensburg, Regensburg, Germany

    Tilman Heise

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Kim, H.J., Chaulk, S., Arthur, D., Edwards, R.A., Glover, J.N.M. (2020). Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones. In: Heise, T. (eds) RNA Chaperones. Methods in Molecular Biology, vol 2106. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0231-7_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0231-7_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0230-0

  • Online ISBN: 978-1-0716-0231-7

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