Abstract
Tetramers are a powerful tool for identification of T cell subsets that are restricted by specific antigen presenting molecules and their cognate antigens. The generation of T cell clones from specific T cell subsets allows for further investigation of the phenotype and function of these cells. Here, we describe a method for sorting and cloning of MR1-restricted T cells using the MR1/5-OP-RU tetramer. This protocol can be easily modified to enrich for expansion of specific or unique subsets of MR1-restricted T cell clones from any tissue to further characterize the phenotype and function of those cells.
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Cansler, M., Null, M., Meermeier, E., Swarbrick, G., Lewinsohn, D., Harriff, M. (2020). Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells. In: Kaipe, H., Magalhaes, I. (eds) MAIT Cells. Methods in Molecular Biology, vol 2098. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0207-2_15
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DOI: https://doi.org/10.1007/978-1-0716-0207-2_15
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0206-5
Online ISBN: 978-1-0716-0207-2
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