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Reprogramming Exosomes for Immunotherapy

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Cell Reprogramming for Immunotherapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2097))

Abstract

Exosomes are nanosized vesicles secreted by nearly all types of cells and play important roles in intercellular communication. Given their unique and important pharmacological properties, exosomes have been emerging as a new class of cell-free therapeutics. Herein, we describe exosomes developed against epidermal growth factor receptor (EGFR), a key factor in epithelial malignancies, involved in enhanced tumor growth, invasion, and metastasis. The exosomes are genetically modified for displaying two distinct types of monoclonal antibodies on the exosome surface, resulting in novel synthetic multivalent antibodies retargeted exosomes (SMART-Exos) that can simultaneously target tumor-associated human EGFR and T-cell surface CD3 receptor. By redirecting and activating T cells toward attacking EGFR-expressing cancer cells, the designed SMART-Exos exhibit highly potent and specific antitumor activity. In this chapter, the methodologies are outlined for generating and using SMART-Exos for cancer immunotherapy.

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Acknowledgments

This work was supported, in part, by University of Southern California School of Pharmacy Start-Up Fund for New Faculty, University of Southern California Ming Hsieh Institute for Engineering Medicine for Cancer, American Association of Pharmaceutical Scientists (AAPS) Foundation New Investigator Grant (to Y.Z.), STOP CANCER Research Career Development Award (to Y.Z.), PhRMA Foundation Research Starter Grant in Translational Medicine and Therapeutics (to Y.Z.), P30CA014089 to the USC Norris Comprehensive Cancer Center, and P30DK048522 to the USC Research Center for Liver Diseases.

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Authors and Affiliations

  1. Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, USA

    Qinqin Cheng, Xiaojing Shi & Yong Zhang

  2. Department of Chemistry, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA, USA

    Yong Zhang

  3. Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, USA

    Yong Zhang

  4. Research Center for Liver Diseases, University of Southern California, Los Angeles, CA, USA

    Yong Zhang

Authors
  1. Qinqin Cheng
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  2. Xiaojing Shi
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  3. Yong Zhang
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Corresponding author

Correspondence to Yong Zhang .

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Editors and Affiliations

  1. Department of Pathology, Yale School of Medicine, New Haven, CT, USA

    Samuel G. Katz

  2. Department of Pathology, Yale School of Medicine, New Haven, CT, USA

    Peter M. Rabinovich

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Cheng, Q., Shi, X., Zhang, Y. (2020). Reprogramming Exosomes for Immunotherapy. In: Katz, S., Rabinovich, P. (eds) Cell Reprogramming for Immunotherapy. Methods in Molecular Biology, vol 2097. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0203-4_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0203-4_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0202-7

  • Online ISBN: 978-1-0716-0203-4

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