HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of Online Monitoring in Shake Flasks (RAMOS and SFR)

  • Tibor Anderlei
  • Michael V. Keebler
  • Jordi Joan Cairó
  • Martí LecinaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2095)


The platforms for bioprocess development have been developed in parallel to the needs of the manufacturing industry of biopharmaceuticals, aiming to ensure the quality and safety of their products. In this sense, Quality by Design (QbD) and Process Analytical Technology (PAT) have become the pillars for quality control and quality assurance.

A new combination of Shake Flask Reader (SFR) and Respiration Activity Monitoring System for online determination of OTR and CTR (RAMOS) allows online monitoring of main culture parameters needed for bioprocess development (pH, pO2, OTR, CTR, and QR) as presented below. Eventually, a case study of the application of the combination of SFR-RAMOS system is presented. The case study discloses the optimization of HEK293 cells cultures through the manipulation of their metabolic behavior.

Key words

Ramos SFR OTR Bioprocess optimization HEK293 cells Culture monitoring Glucose and lactate co-consumption Metabolic phases 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Tibor Anderlei
    • 1
  • Michael V. Keebler
    • 2
  • Jordi Joan Cairó
    • 3
  • Martí Lecina
    • 3
    • 4
    Email author
  1. 1.Adolf Kühner AGBirsfeldenSwitzerland
  2. 2.Kuhner Shaker Inc.San CarlosUSA
  3. 3.Department of Chemical, Biological and Environmental EngineeringUniversitat Autònoma de BarcelonaCerdanyola del VallèsSpain
  4. 4.Bioengineering Department, IQSUniversitat Ramon LlullBarcelonaSpain

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