Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications

  • Vincent Wiegmann
  • Cristina Bernal Martinez
  • Frank BaganzEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2095)


Micro-bioreactors appear frequently in today’s biotechnology industry as screening and process development tools for cell culture applications. The micro-bioreactor’s small volume allows for a high throughput, and when compared to other small-scale systems, such as microtiter plates, its measurement and control capabilities offer a much better insight into the bioprocess. Applikon’s micro-Matrix is one of the micro-bioreactors that are commercially available today. The micro-Matrix system consists of shaken disposable 24 deep square well plates in which each well is controlled individually for pH, dissolved oxygen (DO), and temperature. Additionally, a feeding module supports automated additions of liquid to each well. This chapter describes how the micro-Matrix can be used for fed-batch cultivations of Chinese Hamster Ovary (CHO) cells.

Key words

Miniature shaken bioreactor Micro-Matrix Fed batch GS-CHO cells Scale-down 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Vincent Wiegmann
    • 1
  • Cristina Bernal Martinez
    • 2
  • Frank Baganz
    • 1
    Email author
  1. 1.Department of Biochemical Engineering, The Advanced Centre for Biochemical EngineeringUniversity College LondonLondonUK
  2. 2.Applikon-Biotechnology BVDelftThe Netherlands

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