Advertisement

Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications

  • Vincent Wiegmann
  • Cristina Bernal Martinez
  • Frank BaganzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2095)

Abstract

Micro-bioreactors appear frequently in today’s biotechnology industry as screening and process development tools for cell culture applications. The micro-bioreactor’s small volume allows for a high throughput, and when compared to other small-scale systems, such as microtiter plates, its measurement and control capabilities offer a much better insight into the bioprocess. Applikon’s micro-Matrix is one of the micro-bioreactors that are commercially available today. The micro-Matrix system consists of shaken disposable 24 deep square well plates in which each well is controlled individually for pH, dissolved oxygen (DO), and temperature. Additionally, a feeding module supports automated additions of liquid to each well. This chapter describes how the micro-Matrix can be used for fed-batch cultivations of Chinese Hamster Ovary (CHO) cells.

Key words

Miniature shaken bioreactor Micro-Matrix Fed batch GS-CHO cells Scale-down 

References

  1. 1.
    Doig S, Baganz F, Lye G (2006) High throughput screening and process optimisation. In: Basic Biotechnology, 3rd edn. Cambridge University Press, CambridgeGoogle Scholar
  2. 2.
    Kelly W, Veigne S, Li X, Subramanian SS, Huang Z, Schaefer E (2018) Optimizing performance of semi-continuous cell culture in an ambr15TM microbioreactor using dynamic flux balance modeling. Biotechnol Prog 34(2):420–431CrossRefGoogle Scholar
  3. 3.
    Bareither R, Pollard D (2011) A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need. Biotechnol Prog 27(1):2–14CrossRefGoogle Scholar
  4. 4.
    Hemmerich J, Noack S, Wiechert W, Oldiges M (2018) Microbioreactor systems for accelerated bioprocess development. Biotechnol J 13(4):1700141CrossRefGoogle Scholar
  5. 5.
    Wiegmann V, Martinez CB, Baganz F (2018) A simple method to determine evaporation and compensate for liquid losses in small-scale cell culture systems. Biotechnol Lett 40(7):1029–1036CrossRefGoogle Scholar
  6. 6.
    Hsu WT, Aulakh RPS, Traul DL, Yuk IH (2012) Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors. Cytotechnology 64(6):667–678CrossRefGoogle Scholar
  7. 7.
    Nienow AW, Rielly CD, Brosnan K, Bargh N, Lee K, Coopman K, Hewitt CJ (2013) The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4. Biochem Eng J 76:25–36CrossRefGoogle Scholar
  8. 8.
    Moses S, Manahan M, Ambrogelly A, Ling WLW (2012) Assessment of AMBRTM as a model for high-throughput cell culture process development strategy. Adv Biosci Biotechnol 7:918–927CrossRefGoogle Scholar
  9. 9.
    Chen A, Chitta R, Chang D, Amanullah A (2009) Twenty-four well plate miniature bioreactor system as a scale-down model for cell culture process development. Biotechnol Bioeng 102(1):148–160CrossRefGoogle Scholar
  10. 10.
    Applikon (2016) Brochure: micro-Matrix 24 bioreactors in a convenient microtiter forma [Online]. http://www.applikon-biotechnology.us/images/download/micro-matrix/micro-Matrix-leaflet.pdf. Accessed 30 Jan 2017
  11. 11.
    Lattermann C, Büchs J (2015) Microscale and miniscale fermentation and screening. Curr Opin Biotechnol 35:1–6CrossRefGoogle Scholar
  12. 12.
    DePalma A (2014) Single-use bioreactors dare to scale. Gen Eng Biotechnol News 14:24–26–27Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Vincent Wiegmann
    • 1
  • Cristina Bernal Martinez
    • 2
  • Frank Baganz
    • 1
    Email author
  1. 1.Department of Biochemical Engineering, The Advanced Centre for Biochemical EngineeringUniversity College LondonLondonUK
  2. 2.Applikon-Biotechnology BVDelftThe Netherlands

Personalised recommendations