Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications
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Micro-bioreactors appear frequently in today’s biotechnology industry as screening and process development tools for cell culture applications. The micro-bioreactor’s small volume allows for a high throughput, and when compared to other small-scale systems, such as microtiter plates, its measurement and control capabilities offer a much better insight into the bioprocess. Applikon’s micro-Matrix is one of the micro-bioreactors that are commercially available today. The micro-Matrix system consists of shaken disposable 24 deep square well plates in which each well is controlled individually for pH, dissolved oxygen (DO), and temperature. Additionally, a feeding module supports automated additions of liquid to each well. This chapter describes how the micro-Matrix can be used for fed-batch cultivations of Chinese Hamster Ovary (CHO) cells.
Key wordsMiniature shaken bioreactor Micro-Matrix Fed batch GS-CHO cells Scale-down
- 1.Doig S, Baganz F, Lye G (2006) High throughput screening and process optimisation. In: Basic Biotechnology, 3rd edn. Cambridge University Press, CambridgeGoogle Scholar
- 10.Applikon (2016) Brochure: micro-Matrix 24 bioreactors in a convenient microtiter forma [Online]. http://www.applikon-biotechnology.us/images/download/micro-matrix/micro-Matrix-leaflet.pdf. Accessed 30 Jan 2017
- 12.DePalma A (2014) Single-use bioreactors dare to scale. Gen Eng Biotechnol News 14:24–26–27Google Scholar