Abstract
Assay ready cells are cryopreserved at a highly functional state and can be used in cell-based assay without prior cultivation or cell passaging. Basically, like any other reagent, the cells are applied to the assay instantly after thawing. Introduced initially in the drug discovery process where assay ready Frozen Instant Cells help to streamline cell-based high-throughput screening campaigns, the methodology now has been accepted for a much broader scope of applications and industries. The preparation of assay ready Frozen Instant Cells is not so much a piece of magic but a combination of good cell culture practice, careful handling, and individually optimized cryopreservation protocols. Here, a standard protocol is presented, how HepG2 cells are frozen in an assay ready quality to be used as a reagent in routine cell-based assays.
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References
Allen DD, Caviedes R, Cárdenas AM, Shimahara T, Segura-Aguilar J, Caviedes PA (2005) Cell lines as in vitro models for drug screening and toxicity studies. Drug Dev Ind Pharm 31(8):757–768
Poteser M (2017) Cell-based in vitro models in environmental toxicology: a review. Biomon 4:11–26
Yasuka T, Shinji O, Takumi M (2011) Establishment of a new cell-based assay to measure the activity of sweeteners in fluorescent food extracts. J Agr Food Chem 59(22):12131–12138
Honzel D, Carter SG, Redman KA, Schauss AG, Enders JR, Jensen GS (2008) Comparison of chemical and cell-based antioxidant methods for evaluation of foods and natural products: generating multifaceted data by parallel testing using erythrocytes and polymorphonuclear cells. J Agr Food Chem 56(18):8319–8325
Rust A, Doran C, Hart R, Binz T, Stickings P, Sesardic D, Peden AA, Davletov B (2017) A cell line for detection of botulinum neurotoxin type B. Front pharmacol 8:796
Edwards A, Roscoe L, Longmore C, Bailey F, Sim B, Treasure C (2018) Adaptation of the human Cell Line Activation Test (h-CLAT) to animal-product-free conditions. ALTEX 35(4):477–488
Levy W, Schramm KW, Mertes F, Henkelmann B, Maywald M, Uciechowski P, Loa A, Haedrich J, Thiem I, Hollert H, Goerlich R, Bernsmann T, Rink L (2018) Development and validation of a ready to use cryo-EROD assay for the standardized screening of dioxins and dioxin-like compounds in foodstuffs. Food Chem Toxicol 122(12):206–214
Zhang Z, Guan N, Li T, Mais DE, Wang M (2012) Quality control of cell based high-throughput drug screening. Acta Pharm Sinica B 2(5):429–438
Coecke S, Balls M, Bowe G, Davis J, Gstraunthaler G, Hartung T, Hay R, Merten OW, Price A, Schechtman L, Stacey G, Stokes W (2005) Guidance on good cell culture practice. ALTA 33:261–287
Robinson CJ, Sadick M, Deming SN, Estdale S, Bergelson S, Little L (2014) Assay acceptance criteria for multiwell-plate–based biological potency assays. BioProc Int 12(1):30–41
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Wehmeier, O., Loa, A. (2020). Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 2095. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0191-4_2
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DOI: https://doi.org/10.1007/978-1-0716-0191-4_2
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0191-4
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