Plant-Associated Microbes Alter Root Growth by Modulating Root Apical Meristem

Part of the Methods in Molecular Biology book series (MIMB, volume 2094)


Rhizobacteria are known to produce a variety of signal molecules which may modify plant growth by interfering with phytohormone balance. Among the microbial signals are phytohormones, known to contribute to plant endogenous pool of phytohormones. The current chapter describes different methods to study the regulation of gene expression in root apical meristem in response to rhizobacterial inoculation. We describe protocol for the detection of in planta modulation of CKs and IAA by rhizobacteria and their impact on root growth, dissecting the underlying plant signaling pathway by RNA sequencing.

Key words

RAM Cytokinins Auxin Rhizobacteria Root growth 


  1. 1.
    Sheridan C, Depuydt P, De Ro M, Petit C, Van Gysegem E, Delaere P, Dixon M, Stasiak M, Aciksöz SB, Frossard E et al (2017) Microbial community dynamics and response to plant growth-promoting microorganisms in the rhizosphere of four common food crops cultivated in hydroponics. Microb Ecol 73:378–393CrossRefGoogle Scholar
  2. 2.
    Lawlis T, Parker J, Green A (2017) The effects of auxin on plant growth to apical meristem and varying effects of light intensities. The effects of auxin on plant growth to apical meristem and varying effects of light intensitiesGoogle Scholar
  3. 3.
    Miwa H, Kinoshita A, Fukuda H, Sawa S (2009) Plant meristems: CLAVATA3/ESR-related signaling in the shoot apical meristem and the root apical meristem. J Plant Res 122:31–39CrossRefGoogle Scholar
  4. 4.
    García-Gómez ML, Azpeitia E, Álvarez-Buylla ER (2017) A dynamic genetic-hormonal regulatory network model explains multiple cellular behaviors of the root apical meristem of Arabidopsis thaliana. PLoS Comput Biol 13:e1005488CrossRefGoogle Scholar
  5. 5.
    De Tullio MC, Jiang K, Feldman LJ (2010) Redox regulation of root apical meristem organization: connecting root development to its environment. Plant Physiol Biochem 48:328–336CrossRefGoogle Scholar
  6. 6.
    Malamy JE, Benfey PN (1997) Organization and cell differentiation in lateral roots of Arabidopsis thaliana. Development 124:33–44PubMedGoogle Scholar
  7. 7.
    Palovaara J, de Zeeuw T, Weijers D (2016) Tissue and organ initiation in the plant embryo: a first time for everything. Annu Rev Cell Dev Biol 32:47–75CrossRefGoogle Scholar
  8. 8.
    Ji H, Wang S, Li K, Dr S, Koncz C, Li X (2015) PRL1 modulates root stem cell niche activity and meristem size through WOX5 and PLTs in Arabidopsis. Plant J 81(3):399–412CrossRefGoogle Scholar
  9. 9.
    Fisher AP, Sozzani R (2016) Uncovering the networks involved in stem cell maintenance and asymmetric cell division in the Arabidopsis root. Curr Opin Plant Biol 29:38–43CrossRefGoogle Scholar
  10. 10.
    Bizet F, Hummel I, Bogeat-Triboulot M-B (2014) Length and activity of the root apical meristem revealed in vivo by infrared imaging. J Exp Bot 66:1387–1395CrossRefGoogle Scholar
  11. 11.
    Sozzani R, Iyer-Pascuzzi A (2014) Postembryonic control of root meristem growth and development. Curr Opin Plant Biol 17:7–12CrossRefGoogle Scholar
  12. 12.
    Müller J, Toev T, Heisters M, Teller J, Moore KL, Hause G, Dinesh DC, Katharina B, Abel S (2015) Iron-dependent callose deposition adjusts root meristem maintenance to phosphate availability. Dev Cell 33:216–230CrossRefGoogle Scholar
  13. 13.
    Hussain A, Hasnain S (2011) Interactions of bacterial cytokinins and IAA in the rhizosphere may alter phytostimulatory efficiency of rhizobacteria. World J Microbiol Biotechnol 27:2645–2654CrossRefGoogle Scholar
  14. 14.
    Pérez-Flores P, Valencia-Cantero E, Altamirano-Hernández J, Pelagio-Flores R, López-Bucio J, García-Juárez P, Macías-Rodríguez L (2017) Bacillus methylotrophicus M4-96 isolated from maize (Zea mays) rhizoplane increases growth and auxin content in Arabidopsis thaliana via emission of volatiles. Protoplasma:1–13Google Scholar
  15. 15.
    Wang J, Zhang Y, Li Y, Wang X, Nan W, Hu Y, Zhang H, Zhao C, Wang F, Li P et al (2014) Endophytic microbes Bacillus sp. LZR216-regulated root development is dependent on polar auxin transport in Arabidopsis seedlings. Plant Cell Rep 34:1075–1087CrossRefGoogle Scholar
  16. 16.
    Verma SK, Kingsley K, Bergen M, English C, Elmore M, Kharwar RN, White JF (2017) Bacterial endophytes from rice cut grass (Leersia oryzoides L.) increase growth, promote root gravitropic response, stimulate root hair formation, and protect rice seedlings from disease. Plant Soil:1–16Google Scholar
  17. 17.
    Hussain A, Ullah I, Hasnain S (2017) Microbial manipulation of auxins and cytokinins in plants. Aux Cyto Plant Biol:61–72Google Scholar
  18. 18.
    Ioio RD, Nakamura K, Moubayidin L, Perilli S, Taniguchi M, Morita MT, Aoyama T, Costantino P, Sabatini S (2008) A genetic framework for the control of cell division and differentiation in the root meristem. Science 322:1380–1384CrossRefGoogle Scholar
  19. 19.
    Martin LBB, Nicolas P, Matas AJ, Shinozaki Y, Catalá C, Rose JKC (2016) Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling. Nat Protoc 11:2376–2388CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Botany, Garden CampusAbdul Wali Khan UniversityMardanPakistan
  2. 2.Department of Environmental ScienceIslamic International University IslamabadIslamabadPakistan
  3. 3.Department of Life and Environmental Sciences, College of Natural and Health SciencesZayed UniversityAbu DhabiUAE
  4. 4.Department of Bioinformatics, Biocenter, Functional Genomics and Systems Biology GroupUniversity of WürzburgWürzburgGermany

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