Abstract
The bread wheat genome is large (17 Gb), allohexaploid, and highly repetitive (80–90% of the genome), which makes genomic and epigenomic analyses expensive to conduct and a challenge to analyze. Here we provide an overview of recent bioinformatic and experimental methods that have been developed to understand DNA methylation patterns in the complex polyploid genome of wheat.
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Acknowledgments
This chapter was planned and written by L. J. G. with editorial assistance from P. M. and C. S. The research presented to develop this protocol was supported by the BBSRC via an ERA-CAPS grant (BB/N005104/1) (L.G.) and a BBSRC grant BB/L011786/1 (L.O.).
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Gardiner, LJ. (2020). Understanding DNA Methylation Patterns in Wheat. In: Spillane, C., McKeown, P. (eds) Plant Epigenetics and Epigenomics . Methods in Molecular Biology, vol 2093. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0179-2_3
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DOI: https://doi.org/10.1007/978-1-0716-0179-2_3
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