Abstract
In this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one of the most frequently adulterated. One of the most frequent adulteration is by adding to saffron stigmas different parts of the saffron flower itself to increase volumes. While DNA is the same in all the parts of the plant, the epigenetic state can vary according to the organ and/or tissue of origin, making it possible to discriminate the stigmas from the other parts of saffron flower. In the subsequent method, the protocol to carry out a MS-AFLP analysis of saffron DNA methylation patterns is described.
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Acknowledgments
This work has been partially supported by the European Union COST FA1101 Action “Omics Technologies for Crop Improvement, Traceability, Determination of Authenticity, Adulteration and Origin in Saffron” and the Spanish project INIA RFP2014-00012 “Conservation and management of germplasm collections of the Spanish Network in Bank of Plant Germplasm of Cuenca.”
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Busconi, M., Soffritti, G., Mozos Pascual, M.D., Fernandez, J.A. (2020). Epigenetic Barcodes for Detection of Adulterated Plants and Plant-Derived Products. In: Spillane, C., McKeown, P. (eds) Plant Epigenetics and Epigenomics . Methods in Molecular Biology, vol 2093. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0179-2_16
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DOI: https://doi.org/10.1007/978-1-0716-0179-2_16
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