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Retinal Development pp 109–122Cite as

A Simple Guide for Generating BAC Transgenic Animals for Retinal Research

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2092))

Abstract

Bacterial artificial chromosomes (BACs) are genomic tools that can carry several hundred kilobases of exogenous genomic material. This allows to incorporate sufficiently large DNA stretches to include most if not all upstream and downstream cis-regulatory elements of a gene in order to mimic and analyze its endogenous regulation of expression using a reporter protein in vivo. Here, we illustrate the generation of a BAC:LIF-EGFP transgenic mouse line to describe a simplified version of BAC transgenesis using galK-based recombineering.

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Acknowledgments

We would like to thank Dr. Magdalena Anna Krzyzaniak (Institute of Biochemistry, ETH Zurich) for sharing reagents for recombineering and Dr. Pawel Pelczar (Institute for Laboratory Animal Science, University of Zurich) for pronuclear injections.

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Authors and Affiliations

  1. Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Zurich, Switzerland

    Cavit Agca & Christian Grimm

  2. Molecular Biology, Genetics and Bioengineering Program, Sabanci University, Istanbul, Turkey

    Cavit Agca

  3. Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland

    Christian Grimm

  4. Neuroscience Center (ZNZ), University of Zurich, Zurich, Switzerland

    Christian Grimm

  5. Nanotechnology Research and Application Center (SUNUM), Sabanci University, Istanbul, Turkey

    Cavit Agca

Authors
  1. Cavit Agca
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  2. Christian Grimm
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Corresponding author

Correspondence to Cavit Agca .

Editor information

Editors and Affiliations

  1. Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA

    Chai-An Mao

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Agca, C., Grimm, C. (2020). A Simple Guide for Generating BAC Transgenic Animals for Retinal Research. In: Mao, CA. (eds) Retinal Development. Methods in Molecular Biology, vol 2092. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0175-4_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0175-4_9

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0174-7

  • Online ISBN: 978-1-0716-0175-4

  • eBook Packages: Springer Protocols

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