Abstract
Bacterial artificial chromosomes (BACs) are genomic tools that can carry several hundred kilobases of exogenous genomic material. This allows to incorporate sufficiently large DNA stretches to include most if not all upstream and downstream cis-regulatory elements of a gene in order to mimic and analyze its endogenous regulation of expression using a reporter protein in vivo. Here, we illustrate the generation of a BAC:LIF-EGFP transgenic mouse line to describe a simplified version of BAC transgenesis using galK-based recombineering.
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Acknowledgments
We would like to thank Dr. Magdalena Anna Krzyzaniak (Institute of Biochemistry, ETH Zurich) for sharing reagents for recombineering and Dr. Pawel Pelczar (Institute for Laboratory Animal Science, University of Zurich) for pronuclear injections.
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Agca, C., Grimm, C. (2020). A Simple Guide for Generating BAC Transgenic Animals for Retinal Research. In: Mao, CA. (eds) Retinal Development. Methods in Molecular Biology, vol 2092. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0175-4_9
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DOI: https://doi.org/10.1007/978-1-0716-0175-4_9
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