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Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants

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Retinal Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2092))

Abstract

Activity is important for neural development and regeneration. Enhancing neural activity can facilitate axon regrowth of retinal ganglion cells. Here, we describe various methods, including electrical stimulation, pharmacological manipulation, and optogenetics, to elevate neural activity of retinal explants in mice and to analyze their effects on promoting neurite outgrowth in organotypic culture.

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Acknowledgments

We thank Dr. Chih-Tien Wang for kindly providing us with the recipe for culturing retinal explants. We also thank Dr. Ron Meyer and Ms. Jill Miotke for providing us with their laboratory protocols for the fixation and immunostaining of retinal explants. We appreciate Mr. Yueh-Chun Tsai in assisting us to assemble the Arduino microcontroller board for controlling blue light stimulation. This project was supported by the Ministry of Science and Technology in Taiwan, MOST-107-2311-B-007-002-MY3 (to C.C.C.).

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Authors and Affiliations

  1. Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu, Taiwan

    Chuan-Chin Chiao & Chin-I Lin

  2. Natural and Medical Sciences Institute, University of Tübingen, Reutlingen, Germany

    Meng-Jung Lee

  3. Multi Channel Systems GmbH, Reutlingen, Germany

    Meng-Jung Lee

  4. Graduate Training Centre of Neuroscience, University of Tübingen, Tübingen, Germany

    Meng-Jung Lee

Authors
  1. Chuan-Chin Chiao
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  2. Chin-I Lin
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  3. Meng-Jung Lee
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Corresponding author

Correspondence to Chuan-Chin Chiao .

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Editors and Affiliations

  1. Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA

    Chai-An Mao

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Chiao, CC., Lin, CI., Lee, MJ. (2020). Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants. In: Mao, CA. (eds) Retinal Development. Methods in Molecular Biology, vol 2092. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0175-4_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0175-4_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0174-7

  • Online ISBN: 978-1-0716-0175-4

  • eBook Packages: Springer Protocols

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