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Targeting Enzymes for Pharmaceutical Development pp 179–189Cite as

Enzymatic Bioautographic Methods

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2089))

Abstract

Enzymatic bioautography enables the detection of enzyme inhibitors absorbed on a thin-layer chromatography plate. Therefore, it is an assay format that is particularly useful for the detection of inhibitors present in complex mixtures. The inhibition properties of compounds separated by thin-layer chromatography can be directly analyzed to produce an inhibition profile. Here, we describe the conditions to detect inhibitor of the enzymes xanthine oxidase and β-glucosidase immobilized on agar gel.

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Author information

Authors and Affiliations

  1. Facultad de Ciencias Bioquímicas, Universidad Nacional de Rosario-CONICET, Rosario, Argentina

    I. Ayelen Ramallo, Mario O. Salazar & Ricardo L. E. Furlan

Authors
  1. I. Ayelen Ramallo
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  2. Mario O. Salazar
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  3. Ricardo L. E. Furlan
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Corresponding author

Correspondence to Ricardo L. E. Furlan .

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Editors and Affiliations

  1. Department of Biotechnology, Agricultural University of Athens, Athens, Greece

    Nikolaos E. Labrou

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Ramallo, I.A., Salazar, M.O., Furlan, R.L.E. (2020). Enzymatic Bioautographic Methods. In: Labrou, N. (eds) Targeting Enzymes for Pharmaceutical Development. Methods in Molecular Biology, vol 2089. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0163-1_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0163-1_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0162-4

  • Online ISBN: 978-1-0716-0163-1

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