Abstract
Differential mobility spectrometry (DMS) is capable of separating molecules based on their size and shape. When coupled to mass spectrometry (MS), DMS reduces chemical background and enhances signal-to-noise (S/N) ratio. Flow injection analysis (FIA) is a technique used to introduce samples into the source of the DMS-MS platform. Here we describe the application of FIA-DMS-MS/MS for the analysis of urinary acylcarnitine species. More than 20 acylcarnitine species can be detected and quantified during a single FIA-DMS-MS/MS acquisition.
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Acknowledgments
This work was funded by the Internal Medicine Research Unit (IMRU), within the Pfizer Global Research and Development Business Unit, at Pfizer.
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Vera, N.B., Clasquin, M., Coy, S.L., Vouros, P. (2020). The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines. In: Paglia, G., Astarita, G. (eds) Ion Mobility-Mass Spectrometry . Methods in Molecular Biology, vol 2084. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0030-6_5
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DOI: https://doi.org/10.1007/978-1-0716-0030-6_5
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