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Preparing the Hydrocarbon/Crude Oil

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Hydrocarbon and Lipid Microbiology Protocols

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Abstract

Crude oils are complex mixtures, and a fraction of them can evaporate quite rapidly when oil is spilled into the biosphere. A fraction can also dissolve in underlying water. This article discusses artificially weathering crude oils and refined products, and preparing water-accommodated fractions and true solutions of hydrocarbons. It also discusses some essential precautions for reproducibly adding oils to experimental systems.

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Author information

Authors and Affiliations

  1. ExxonMobil Biomedical Sciences, Inc., Annandale, NJ, 08801, USA

    Roger C. Prince, Josh D. Butler, Gail E. Bragin, Aaron D. Redman, Barbara A. Kelley & Daniel J. Letinski

  2. ExxonMobil Biomedical Sciences, Inc., Houston, TX, 77002, USA

    Thomas F. Parkerton

Authors
  1. Roger C. Prince
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  2. Josh D. Butler
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  3. Gail E. Bragin
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  4. Thomas F. Parkerton
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  5. Aaron D. Redman
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  6. Barbara A. Kelley
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  7. Daniel J. Letinski
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Corresponding author

Correspondence to Roger C. Prince .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Braunschweig, Germany

    Kenneth N. Timmis

  3. Dept. Biologia, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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Prince, R.C. et al. (2016). Preparing the Hydrocarbon/Crude Oil. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2016_220

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  • DOI: https://doi.org/10.1007/8623_2016_220

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-53107-5

  • Online ISBN: 978-3-662-53108-2

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