Abstract
Fungi form the biggest microbial biomass and are key drivers of hydrocarbon biodegradation. We here describe standard cultivation methods for fungi that use hydrocarbons either as sole carbon and energy source or co-metabolically degrade them. The methods include both liquid (Protocols 1 and 2) and solid-state cultivation approaches (Protocol 3) as well as a versatile protocol to monitor fungal biomass based on the quantification of the specific fungal marker ergosterol (Protocol 4).
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Schlosser, D., Wick, L.Y. (2015). Cultivation of Hydrocarbon-Degrading Fungi. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2015_97
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DOI: https://doi.org/10.1007/8623_2015_97
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