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In Vitro High-Pressure Incubation and Activity Measurement of Deep-Sea Methanogenic Archaea

  • Eiji Tasumi
  • Katsunori Yanagawa
  • Junichi Miyazaki
  • Ken Takai
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

A high-pressure cultivation or incubation system can reproduce elevated in situ pressure conditions of deep-sea environments in a laboratory. It has significantly contributed to the piezophysiological studies of microorganisms, especially psychrophilic heterotrophs, since the first report of high-pressure incubation of deep-sea microorganisms many decades ago. The deep-sea microorganisms growing on gaseous substrates such as H2, CH4, N2, and CO2 play important roles in biogeochemical cycles around gas-rich deep-sea environments such as deep-sea hydrothermal vent habitats. However, due to the difficulties in obtaining pressure-proof gastight high-pressure cultivation systems for increased gaseous substrates and the tricky way of their operations, only a few gas-utilizing microorganisms have been investigated under high-pressure conditions so far. Here, we describe the protocols for high-pressure microbiological experiments with easy handling systems that enable the high-pressure cultivation of hyperthermophilic hydrogenotrophic methanogens and the following highly sensitive measurement of methanogenesis activity in the deep-sea sediments using radiolabeled tracers under high-pressure conditions.

Keywords:

Activity measurement High-pressure incubation Methanogenesis 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Eiji Tasumi
    • 1
  • Katsunori Yanagawa
    • 1
  • Junichi Miyazaki
    • 1
  • Ken Takai
    • 1
  1. 1.Department of Subsurface Geobiological Analysis and Research (D-SUGAR)Japan Agency for Marine-Earth Science and Technology (JAMSTEC)YokosukaJapan

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