Cultivation of Methylotrophs

  • Donovan P. Kelly
  • Julie K. Ardley
  • Ann P. Wood
Part of the Springer Protocols Handbooks book series (SPH)


Methylotrophy is the ability of numerous Alpha-, Beta-, and Gammaproteobacteria, Gram-positive bacteria, Actinomycetes, and yeasts to obtain all the energy and carbon required for growth from the metabolism of methyl compounds which have no carbon–carbon bonds. Numerous media and techniques for the isolation and culture of methylotrophs have been described, and a comprehensive description of these protocols is given, with substrates such as methanol, methylamines, and methylated sulfur compounds, and the description of some more complex media. The metabolic and habitat diversity of methylotrophs is outlined, ranging from soil and freshwater organisms, through thermophiles and yeasts, to animal hosts and plant colonisers, including specialised types that induce (and reside in) root nodules in some plant species. The methods for the commercial exploitation of some methylotrophs are outlined. This survey excludes the methanotrophs, growing primarily on gaseous methane, as the cultivation of these is considered elsewhere.


Bacillus Hyphomicrobium Methanol Methylamines Methylated sulfur compounds Methylobacterium Plants Proteobacteria Rhizobia Yeast 



We thank all the co-authors of the ‘Working with Rhizobia’ manual, whose work has contributed to the rhizobial section of this chapter: Abdullahi Bala, Rosalind Deaker, Michael Dilworth, Ken Giller, David Herridge, John Howieson, Mariangela Hungria, Nancy Karanja, Vanessa Melino, Sofie De Meyer, Graham O’Hara, Phil Poole, Wayne Reeve, Ricardo Silva Araujo, Ravi Tiwari, Anabel Vivas-Marfisi, Paul Woomer, and Ron Yates.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Donovan P. Kelly
    • 1
  • Julie K. Ardley
    • 2
  • Ann P. Wood
    • 3
  1. 1.School of Life Sciences, University of WarwickCoventryUK
  2. 2.Centre for Rhizobium Studies, School of Veterinary and Life SciencesMurdoch UniversityMurdochAustralia
  3. 3.Department of Biochemistry, Henriette Raphael HouseKing’s College LondonLondonUK

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