Protocols for Subtidal and Deep-Sea Benthic Oil Spill Simulations

  • Philippe Cuny
  • Vincent Grossi
  • Cécile Militon
  • Christian Tamburini
  • Georges Stora
  • Franck Gilbert
Part of the Springer Protocols Handbooks book series (SPH)


In addition to weathering, physical interactions between oil and suspended particulate matter play a role in the dispersal and sedimentation of spilled oil in marine ecosystems. In coastal regions characterized by high concentrations of suspended particulate material, up to 30% of the total spill may reach the seafloor where the oil can interact with biological and chemical sedimentary processes. Simulating and understanding the effects and dynamics of spilled oil in subtidal and deeper sediments represents a challenge in microbial ecology and biogeochemistry. However, setting up and monitoring experimental devices in areas permanently covered with seawater is indeed more complicated than in intertidal areas because the access is limited and requires adapted equipment. Here, we describe simple but efficient protocols for subtidal benthic oil spill simulation based on the use of plastic corers, as well as the characteristics of a benthic device enabling the in situ incubation of oiled deep-sea sediments. Associated logistic efforts and requirements are also presented.


Benthic incubation device Biodegradation Bioturbation Oil dynamics Plastic corers 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Philippe Cuny
    • 1
  • Vincent Grossi
    • 2
  • Cécile Militon
    • 1
  • Christian Tamburini
    • 1
  • Georges Stora
    • 1
  • Franck Gilbert
    • 3
    • 4
  1. 1.Aix-Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110Marseille cedex 9France
  2. 2.Laboratoire de Geologie de LyonUniversite Lyon 1, CNRS UMR5276VilleurbanneFrance
  3. 3.EcoLab (Laboratoire écologie fonctionnelle et environnement)Université de Toulouse; INP, UPSToulouseFrance
  4. 4.CNRS, EcoLabToulouseFrance

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