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Use of Bacterial Polyhydroxyalkanoates in Protein Display Technologies

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Hydrocarbon and Lipid Microbiology Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Protein display and immobilization are powerful tools used in industrial biocatalysts, bioremediation, biomolecule screening and purification, as well as biosensor applications. Immobilization can aid in the stability and function of a protein and can allow its recovery and potential reuse. Traditional protein immobilization techniques involving entrapment or non-covalent interactions between the protein and support materials are susceptible to leaching and often require additional cross-linking steps; which may be costly, potentially toxic and may negatively affect the function of the protein. All of these approaches require multiple steps to produce, isolate, and immobilize the protein of interest. Here we present protocols for the in vivo production of a protein of interest covalently immobilized on the surface of a bio-polyester resin in a single step. The steps involved in vector construction, protein/bead production, and isolation are explained and outlined.

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Author information

Authors and Affiliations

  1. Polybatics Ltd., Palmerston North, New Zealand

    Iain D. Hay & Bernd H. A. Rehm

  2. Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand

    David O. Hooks & Bernd H. A. Rehm

  3. MacDiarmid Institute of Advanced Materials and Nanotechnology, Wellington, New Zealand

    Bernd H. A. Rehm

Authors
  1. Iain D. Hay
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  2. David O. Hooks
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  3. Bernd H. A. Rehm
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Corresponding author

Correspondence to Bernd H. A. Rehm .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex Dept. Biological Sciences, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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© 2014 Springer-Verlag Berlin Heidelberg

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Hay, I.D., Hooks, D.O., Rehm, B.H.A. (2014). Use of Bacterial Polyhydroxyalkanoates in Protein Display Technologies. In: McGenity, T., Timmis, K., Nogales, B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2014_34

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  • DOI: https://doi.org/10.1007/8623_2014_34

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-53113-6

  • Online ISBN: 978-3-662-53115-0

  • eBook Packages: Springer Protocols

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