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Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes

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Brain-Derived Neurotrophic Factor (BDNF)

Part of the book series: Neuromethods ((NM,volume 143))

Abstract

BDNF gene has a complex structure and is regulated by multiple time- and stimulus-dependent promoters and distal regulatory elements. To better understand the interplay between these regulatory units, it is beneficial to study BDNF regulation in the context of a larger gene locus. The bacterial artificial chromosome (BAC) system allows propagating up to 300 kb long genomic regions, enabling to study the whole BDNF gene. Here, we present the methods for usage of BACs for studying BDNF gene regulation in primary cultures of rat cortical neurons and astrocytes.

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Authors and Affiliations

  1. Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia

    Kaur Jaanson, Angela Pärn & Tõnis Timmusk

Authors
  1. Kaur Jaanson
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  2. Angela Pärn
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  3. Tõnis Timmusk
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Corresponding author

Correspondence to Tõnis Timmusk .

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Editors and Affiliations

  1. Center for Neuroscience and Cell Biology and Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    Carlos B. Duarte

  2. BRAIN Center for Neuroscience, Department of Life Sciences, University of Trieste, Trieste, Italy

    Enrico Tongiorgi

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Jaanson, K., Pärn, A., Timmusk, T. (2018). Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes. In: Duarte, C., Tongiorgi, E. (eds) Brain-Derived Neurotrophic Factor (BDNF). Neuromethods, vol 143. Humana, New York, NY. https://doi.org/10.1007/7657_2018_10

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  • DOI: https://doi.org/10.1007/7657_2018_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8969-0

  • Online ISBN: 978-1-4939-8970-6

  • eBook Packages: Springer Protocols

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