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pp 1-16 | Cite as

BDNF Function in Long-Term Synaptic Plasticity in the Dentate Gyrus In Vivo: Methods for Local Drug Delivery and Biochemical Analysis of Translation

  • Debabrata PanjaEmail author
  • Clive R. Bramham
Protocol
Part of the Neuromethods book series

Abstract

Neurotrophins are essential for multiple aspects of neuronal development and to important functions like synaptic plasticity. Brain-derived neurotrophic factor (BDNF) is a critical activity-dependent modulator of gene expression which regulates both transcription and translation. BDNF is crucial in the maintenance of long-term potentiation (LTP) at synapses and regulates protein synthesis at the dendritic and synaptic level. To elucidate the mechanisms operating in the hippocampal dentate gyrus region, in vivo electrophysiology and pharmacology is combined with analysis of signaling pathways and protein synthesis. Here, we present methods for the analysis of translation initiation, polysome formation, and translational efficiency in the context of LTP consolidation in live rodents.

Keywords

BDNF Dentate gyrus In vivo electrophysiology Long-term potentiation Polysome profiling Surface sensing of translation (SuNSET) Synaptoneurosomes TrkB 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.National Institute of Mental HealthBethesdaUSA
  2. 2.Department of Biomedicine and KG Jebsen Center for Research on Neuropsychiatric DisordersUniversity of BergenBergenNorway

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