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Isolating and Screening Subcellular miRNAs in Neuron

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MicroRNA Technologies

Part of the book series: Neuromethods ((NM,volume 128))

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Abstract

Since local protein synthesis is proposed to explain spatial and temporal regulation of gene expression in highly polarized cells such as neurons, various mechanisms for regulating protein synthesis are suggested. Among them, microRNA (miRNA) is one of the key regulators for protein synthesis in the synaptic area. As miRNAs can selectively repress mRNA translation with sequence-specific manner, profiling of miRNAs located in synaptic area has been an important topic to understand the function of neuronal miRNAs. Interestingly, many miRNAs are detected in the synaptic area, but their subcellular distribution in neuron varies. This suggests that there are cellular mechanisms actively regulating miRNA expression and localization in subcellular compartments of neurons. In this chapter, we review currently available methods of synaptic miRNA profiling from isolating samples, purifying RNAs, and measuring expression.

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Acknowledgement

This work is supported by Deutsche Forschungsgemeinschaft (German Research Foundation), University of Cologne (Cologne Fortune), and cure SMA.

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Authors and Affiliations

  1. Institute of Human Genetics, University Hospital Cologne, Kerpener Street 34, 50931, Cologne, Germany

    Min Jeong Kye

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  1. Min Jeong Kye
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Correspondence to Min Jeong Kye .

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  1. Institute of Human Genetics, University of Cologne, Cologne, Germany

    Min Jeong Kye

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© 2016 Springer Science+Business Media New York

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Kye, M.J. (2016). Isolating and Screening Subcellular miRNAs in Neuron. In: Kye, M. (eds) MicroRNA Technologies. Neuromethods, vol 128. Humana Press, New York, NY. https://doi.org/10.1007/7657_2016_4

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  • DOI: https://doi.org/10.1007/7657_2016_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7173-2

  • Online ISBN: 978-1-4939-7175-6

  • eBook Packages: Springer Protocols

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