Abstract
Intracranial xenograft meningioma models are considered best because of their biological relevance in tumor location. Traditionally, difficulty in obtaining serial measurements and high costs hindered intracranial tumor research; however, with the development of bioluminescence imaging systems, serial measurements of intracranial xenografted immortal meningioma cell lines can now be completed in a noninvasive, cost-effective, and efficient manner. This method involves transfecting immortal meningioma cells with the firefly luciferase gene, which then expresses the luciferase enzyme. This enzyme oxidizes luciferin in a reaction that releases photon energy that can be easily measured and recorded, even after the transfected tumor cells have been implanted intracranially in a mouse model. The transfected cells replicate key macroscopic, microscopic, immunohistochemical, and ultrastructure features of aggressive meningiomas.
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Acknowledgment
We thank Kristin Kraus, M.Sc., for her excellent editorial assistance.
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Rainey, J., Jensen, R.L. (2012). Meningioma Models. In: Martínez Murillo, R., Martínez, A. (eds) Animal Models of Brain Tumors. Neuromethods, vol 77. Humana Press, Totowa, NJ. https://doi.org/10.1007/7657_2011_27
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DOI: https://doi.org/10.1007/7657_2011_27
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